Composite

Part:BBa_J202016:Design

Designed by: Jiyeon Park   Group: Tagkopoulos Lab   (2012-07-26)


PBAD Characterization (Mutant#1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 801


Design Notes

For characterization with L-arabinose, E.coli strain DH5alpha was used. This strain provides araC on its chromosome. araC auto-regulates its own concentration in the cell.


Source

Built from new part (J202000) and existing parts in the registry though BioBrick assembly.

References